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Lightfest! Advanced Light Microscopy Symposium 2024

May 15 - May 17

About Lightfest!

Join us for the second annual Lightfest! Advanced Light Microscopy Symposium at CNSI. A celebration of UNESCO’s International Day of Light, this event will be held from May 15 – 17, in honor of Theodore Maiman, who fired the first laser right here in southern California on May 16th, 1960.

This symposium will feature invited talks from a selection of light microscopy users, poster presentations and awards, an image contest, and vendor booths. This event is free and open to anyone, we hope to see you there!

Keynote Speaker: Scott E. Fraser, Ph.D. - Provost Professor, Director of Science Initiatives, USC

We are thrilled to announce the 2024 Lightfest Advanced Microscopy Symposium Keynote speaker:

Opening Keynote: Adding Dimensions to Intravital Imaging to Better Eavesdrop on Biology
Scott E. Fraser, Ph.D.
University of Southern California
Wednesday, May 15, 2024

Imaging of living specimens can animate the wealth of high-throughput molecular data to better understand complex events ranging from embryonic development to disease processes.  We are advancing this approach despite the unavoidable tradeoffs – between spatial & temporal resolution, field of view, limited photon budget – by constructing faster and more efficient light sheet and laser-scanning microscopes that maintain subcellular resolution.

Our two-photon light-sheet microscope combines the deep penetration of two-photon microscopy and the speed of light sheet microscopy to generate images with more than 10x improved imaging speed & sensitivity.  Better engineering of the detection objective’s point-spread-function improves this another 3-fold.  Two-photon excitation light is far less scattered, permitting subcellular resolution to be maintained better than conventional light sheet microscopes, resulting in 4D (3D over time) cell and molecular imaging with sufficient speed and resolution to unambiguous trace cell lineages, movements and signals in intact systems.

To increase the 5th Dimension (# of simultaneous labels), we are refining new multispectral image analysis tools that exceed the performance of our previous work on Linear Unmixing by orders of magnitude in speed, error propagation and accuracy.  Novel denoising strategies using machine learning permit imaging at far lower light levels, yielding rapid and unambiguous analyses without perturbing even fragile multiplex-labeled specimens.

Parallel refinements in label-free approaches extend imaging to patient-derived tissues and even human subjects.  The low concentrations of these intrinsic labels required us to refine fluorescence lifetime imaging (FLIM), and combine it with multispectral and advanced denoising tools, to perform intravital imaging in such challenging settings.

Combined, these imaging and analysis tools offer the multi-dimensional imaging required to follow key events in intact systems as they take place, and allow us to use noise and variance as experimental tools rather than experimental limitations.

Schedule

This event is free thanks to the generous support of our sponsors:

Details

Start:
May 15
End:
May 17
Event Categories:
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Website:
https://alms.cnsi.ucla.edu/

Organizer

Advanced Light Microscopy and Spectroscopy Lab
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Venue

California NanoSystems Institute (CNSI)
570 Westwood Plaza
Los Angeles, CA 90095 United States
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Phone
3102674838
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